Storage of Mounted Paraffin Wax Sections
نویسندگان
چکیده
منابع مشابه
Storage of Frozen Sections in Paraffin Blocks.
The development of the techniques for cutting frozen sections, in particular the use of cryostat microtomes (Pearse, 1960; Cunningham, Bitensky, Chayen, and Silcox, 1960 and 1962; Goodbody, 1963 a and b; Cunningham, 1963), has resulted in sections which not only are produced routinely very rapidly but which are completely acceptable histologically. Such sections would be ideal, especially where...
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The problem of producing large nwmbers of sections for teaching purposes and staining them by haematoxylin and eosin has become acute in.the post-war years, due to the shortage both of trained technicians and of reagents, especially ethyl alcohol. Methods previously used have suffered from the disadvantages of being too long, or wasteful of reagents, and it was with these factors in mind that t...
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Aim-To analyse the effect of sectioning on the assessment of karyotypic heterogeneity by interphase cytogenetics in paraffin wax embedded normal squamous epithelium and to apply the principles derived to invasive cervical carcinoma.Methods-Normal male (n = 5) and female (n = 5) squamous epithelia were hybridised with peri-centromeric repeat probes specific for chromosomes X (DXZ1) and 17 (D17Z1...
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Several immunohistological methods for the demonstration of progesterone receptors were tried on routinely processed paraffin wax sections of breast carcinoma, using Abbott's PgR-ICA monoclonal antibody. The best results were obtained with the avidin-biotin-immunoperoxidase complex method with no prior trypsinisation or DNAse digestion, and with imidazole added to the final diaminobenzidine dev...
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A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other publish...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2000
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500057540